As a result of this pursuit new tools are constantly being developed to allow scientists to observe natural phenomena in the most direct and non-invasive way possible. The driving force behind most scientific inquiries is to better understand the natural world we live in. oneidensis MR-1 and the mixed biofilm showed a wide range of compounds that this bacterium uses for the dissimilatory reduction of extracellular metal oxides, including riboflavin, iron-bound heme and heme biosynthetic intermediates, and the siderophore putrebactin.

coelicolor A3(2) provided a means of validation for the method while data from S. Using this technique, we investigated single colonies of Shewanella oneidensis MR-1, Bacillus subtilis 3610, Streptomyces coelicolor A3(2) as well as a mixed biofilm of S. Here we report the use of nanospray desorption electrospray ionization (nanoDESI) imaging mass spectrometry for in vivo metabolic profiling of living bacterial colonies directly from the Petri dish with absolutely no sample preparation needed. However, there is a lack of methodologies that allow the direct investigation of chemical gradients and interactions in living biological systems, in real time. Understanding molecular interaction pathways in complex biological systems constitutes a treasure trove of knowledge that might facilitate the specific, chemical manipulation of the countless microbiological systems that occur throughout our world.

Programs similar to odesi